Nanowerk May 4, 2023 For DNA-based storage systems scalable parallel random access to information needs to be robustly established. An international team of researchers (the Netherlands, USA – Microsoft, University of Washington, UK) has developed a thermoconfined polymerase chain reaction, which enabled multiplexed, repeated random access to compartmentalized DNA files. The strategy was based on localizing biotin-functionalized oligonucleotides inside thermoresponsive, semipermeable microcapsules. At low temperatures, microcapsules were permeable to enzymes, primers, and amplified products, whereas at high temperatures, membrane collapse prevented molecular crosstalk during amplification. Their data showed that the platform outperformed non-compartmentalized DNA storage compared with repeated random access […]
Tag Archives: DNA data storage
The future of data storage is double-helical, research indicates
Science Daily March 3, 2022 A team of researchers in the US (University of Illinois, UMass Amherst, Stanford University) expanded molecular alphabet for DNA data storage comprising four natural and seven chemically modified nucleotides that are readily detected and distinguished using nanopore sequencers. They showed that Mycobacterium smegmatis porin A (MspA) nanopores can accurately discriminate 77 combinations and orderings of chemically diverse monomers within homo- and heterotetrameric sequences. The sequencing accuracy exceeded 60%. The extended molecular alphabet may potentially offer a nearly 2-fold increase in storage density and potentially the same order of reduction in the recording latency, thereby enabling […]
Translation software enables efficient DNA data storage
Nanowerk April 2, 2021 In support of the IARPA Molecular Information Storage (MIST) program researchers at the Los Alamos National Laboratory have developed a software called the Adaptive DNA Storage Codec (ADS Codex), that translates data files from what a computer understands into what biology understands. The short-term goal of MIST is to write 1 terabyte—a trillion bytes—and read 10 terabytes within 24 hours for $1,000. ADS Codex addresses two big obstacles to creating DNA data files: Figured out new strategies for error correction as the error rates while writing to molecular storage; Added additional information called error detection codes […]
Using electricity to increase the amount of data that can be stored by DNA
Phys.org January 12, 2021 Researchers at Columbia University have developed a new electrogenetic framework for direct storage of digital data in living cells. Using an engineered redox-responsive CRISPR adaptation system, they encoded binary data in 3-bit units into CRISPR arrays of bacterial cells by electrical stimulation. They demonstrated multiplex data encoding into barcoded cell populations to yield meaningful information storage and capacity up to 72 bits, which can be maintained over many generations in natural open environments. Their work establishes a direct digital-to-biological data storage framework and advances the capacity for information exchange between silicon- and carbon-based entities…read more. TECHNICAL ARTICLE
Key obstacles to scaling up DNA data storage
Science Daily June 3, 2019 To reach practical capacities, new systems for organizing and accessing information are needed. Researchers at North Carolina State University used chemical handles to selectively extract unique files from a complex database of DNA mimicking 5 TB of data and designed and implemented a nested file address system that increases the theoretical maximum capacity of DNA storage systems by five orders of magnitude. The advancements enable the development and future scaling of DNA-based data storage systems with modern capacities and file access capabilities…read more. TECHNICAL ARTICLES