Phys.org July 12, 2021
To construct a bipedal DNA walking nanomachine an international team of researchers (USA – University of New York, China) constructed a series of DNA logic circuits based on cascade strand displacement amplification. They modified track DNA probes with the stem-loop structure on the electrode interface. In the upstream homogeneous system, target-triggered strand displacement amplification was introduced to generate a large number of single-stranded sequences. The DNA three-way junction structure was then assembled as a bipedal moved around the electrode interface to enrich electrochemical signal molecules. Using incomplete three-way junction and double-stranded structures, logic gates AND and OR were constructed by cascading strand displacement amplification. Next, NAND, NOR, XOR, and XNOR gates were developed. The way three-input AND and OR gates were fabricated using the designed four-way junction and double-stranded structures. These logic circuits be applied in ultra-sensitive biomedical sensing, and they provide new ideas for biomolecule information control, communication, and biological computing…read more. Open Access TECHNICAL ARTICLE
Bipedal DNA walking based electrochemical detection of miRNA. Credit: SIBET