Phys.org July 27, 2021
Researchers in South Korea have developed a fluorescence-based nanoparticle tracking analysis (NTA) system to characterize the size and protein expression of individual extracellular vesicles (EV). In this system, a sheet of lasers with four different wavelengths was shone onto the EVs according to a programmed schedule, providing scattering images intercalated by three fluorescent images. The size, ratio, and scattered light of thousands of individual particles were observed, and a six-dimensional value composed of three different fluorescence was obtained. They measured the distribution of specific proteins in EVs (e.g., exosomes) at individual particle levels and analyzed the relationship between the proteins. Using this system, it is possible to observe and get the data without control group…read more. TECHNICAL ARTICLE
