Phys.org May 26, 2021
Researchers in South Korea have developed a plasmofluidic PCR chip comprising glass nanopillar arrays with Au nanoislands and gas-permeable microfluidic channels, which contain reaction microchamber arrays, a precharged vacuum cell, and a vapor barrier. The on-chip configuration allows both spontaneous sample loading and microbubble-free PCR reaction during which the plasmonic nanopillar arrays result in ultrafast photothermal cycling. After rapid sample loading, two-step PCR results for 40 cycles show rapid amplification in 264 s for lambda-DNA, and 306 s for plasmids expressing SARS-CoV-2 envelope protein. In addition, the in situ cyclic real-time quantification of amplicons clearly demonstrates the amplification efficiencies of more than 91%. This PCR platform can provide rapid point-of-care molecular diagnostics…read more. Open Access TECHNICAL ARTICLEÂ
Nanoplasmonic photothermal modulation in solution phase… Credit: ACS Nano 2021, XXXX, XXX, XXX-XXX, May 19, 2021Â