Science Daily October 3, 2018
An international team of researchers (Japan, the Netherlands) has developed microfluidic paper‐based analytical devices (μPADs) based on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. They use BRET‐based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, fully reagent‐free operation, including on‐device blood plasma separation. It takes about 20 minutes and a drop of blood to test the sample for anti-bodies. The device is ideally suited for user‐friendly point‐of‐care testing in low‐resource environments…read more. Open Access TECHNICAL ARTICLE
Schematic of the LUMABS working principle with the “closed form” green light‐emitting and the “open form” blue light‐emitting protein sensor in the absence and presence of target antibody. Credit: Angewandte Chemie International Edition, 2018; DOI: 10.1002/anie.201808070