Science Daily  May 9, 2023 A team of researchers in the US (University of Oklahoma, Yale University) exposed mammalian cells to metallic nanoparticles and then embedded them within different swellable hydrogels to enable quantitative 3D imaging approaching electron-microscopy-like resolution using a standard light microscope. Using the nanoparticles’ light scattering properties, they demonstrated quantitative label-free imaging of intracellular nanoparticles with ultrastructural context and confirmed the compatibility of two expansion microscopy protocols, protein retention and pan-expansion microscopy, with nanoparticle uptake studies. The relative differences between nanoparticle cellular accumulation for various surface modifications were validated using mass spectrometry and determined the intracellular nanoparticle […]