Phys.org  February 6, 2024 A flawless recording of single-photon spatiotemporal information poses significant challenges for the microscope data acquisition (DAQ) system. An international team of researchers (Italy, Switzerland) has presented a DAQ module based on the digital frequency domain principle, able to record essential spatial and temporal features of photons. They used the module to extend the capabilities of established imaging techniques based on single-photon avalanche diode (SPAD) array detectors. They used the module to introduce a robust multispecies approach encoding the fluorophore excitation spectra in the time domain. By combining time-resolved stimulated emission depletion microscopy with image scanning microscopy, […]