Eurekalert  November 25, 2019 To date, gene-circuit-based sensors have primarily used optical proteins as reporter outputs, which has limited the potential to measure multiple distinct signals. An international team of researchers (Canada, USA – Arizona State University) has engineered a scalable system of reporter enzymes that cleave specific DNA sequences in solution, which results in an electrochemical signal when these newly liberated strands are captured at the surface of a nanostructured microelectrode. They describe the development of this interface and show its utility using a ligand-inducible gene circuit and toehold switch-based sensors by demonstrating the detection of multiple antibiotic resistance […]