Science Daily September 27, 2023 The CRISPR-Cas13 ribonucleases have been widely applied for RNA knockdown and transcriptional modulation owing to their high programmability and specificity. However, the large size of Cas13 effectors and their non-specific RNA cleavage upon target activation limit the adeno-associated virus-based delivery of Cas13 systems for therapeutic applications. Researchers at Rice University descried detailed biochemical and structural characterizations of a compact Cas13 (Cas13bt3) suitable for adeno-associated virus delivery. Distinct from many other Cas13 systems, Cas13bt3 cleaves the target and other nonspecific RNA at internal “UC” sites and was activated in a target length-dependent manner. The cryo-electron microscope […]
Tag Archives: CRISPR
New gene editor harnesses jumping genes for precise DNA integration
Phys.org June 12, 2019 Researchers at Columbia University have developed a technology called INTEGRATE, which harnesses bacterial jumping genes to reliably insert any DNA sequence into the genome without cutting DNA. Rather than introduce DNA breaks and rely on the cell to repair the break, as done in CRISPR, INTEGRATE directly inserts a user-defined DNA sequence at a precise location in the genome. The technique should enable a vast range of new gene editing opportunities in biotechnology, gene and cell therapies, engineered crops, and biologics. The INTEGRATE technology offers a fresh new approach with the same programmability and ease of use […]