Nanowerk  May 4, 2023 For DNA-based storage systems scalable parallel random access to information needs to be robustly established. An international team of researchers (the Netherlands, USA – Microsoft, University of Washington, UK) has developed a thermoconfined polymerase chain reaction, which enabled multiplexed, repeated random access to compartmentalized DNA files. The strategy was based on localizing biotin-functionalized oligonucleotides inside thermoresponsive, semipermeable microcapsules. At low temperatures, microcapsules were permeable to enzymes, primers, and amplified products, whereas at high temperatures, membrane collapse prevented molecular crosstalk during amplification. Their data showed that the platform outperformed non-compartmentalized DNA storage compared with repeated random access […]