Phys/org December 16, 2024
Field-effect transistor (FET)-based electronic biosensing platforms are particularly attractive due to their sensitivity, fast turn-around time, potential for parallel detection of multiple pathogens, and compatibility with semiconductor manufacturing. However, scalability multiplexed biofunctionalization, nanoscale precision for immobilizing different types of pathogen-specific bioreceptors, are unmet. An international team of researchers (USA – New York University, SUNY Downstate Health Sciences University, industry, Italy) proposed a paradigm shift in FET biofunctionalization using thermal scanning probe lithography (tSPL) with a thermochemically sensitive polymer which could be spin-coated on fully fabricated FET chips, making this approach applicable to any FET sensor material and technology. They demonstrated the capability of this method by immobilizing different types of bioreceptors at prescribed locations on a chip paving the way for massively parallel FET detection of multiple pathogens. The sensors achieved ultra-sensitive detection of a minimum measured concentrations of SARS-CoV-2 spike proteins and 10 human SARS-CoV-2 infectious live virus particles per ml… read more. TECHNICAL ARTICLE
Graphical abstract. Credit: Nanoscale, 2024,16, 19620-19632