Phys.org August 30, 2024
Microbial cells often lose the multicopy expression plasmids during long-term cultivations. Because of the advantages related to titers, yields, and productivities plasmid stability is essential for industrially relevant biobased processes. Researchers in the UK designed and tested plasmid addiction systems based on the complementation of essential genes. They stabilized a multicopy plasmid by implementing a plasmid addiction tool based on the complementation of mutants lacking RubisCO, which is essential for CO2 fixation. Expressing the mevalonate pathway operon (MvaES) using this addiction system resulted in the production of carbon yields of ∼25%. According to the researchers the mevalonate titers and yields they obtained using CO2 were the highest achieved to date to produce C6 compounds from C1 feedstocks… read more. Open Access TECHNICAL ARTICLE
Graphical abstract. Credit: ACS Sustainable Chem. Eng., XXXX, XXX, XXX-XXX , August 26, 2024