Phys.org May 9, 2024
Isoprenol is a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane) and is produced in several engineered microorganisms. Recently, Pseudomonas putida has gained interest as a future host for isoprenol bioproduction as it can utilize carbon sources from inexpensive plant biomass. A team of researchers in the US (industry, PNNL, Lawrence Berkeley National Laboratory) engineered metabolically versatile host P. putida for isoprenol production. They employed two computational modeling approaches to predict gene knockout targets and optimize the “IPP-bypass” pathway in P. putida to maximize isoprenol production. Altogether, the highest isoprenol production titer from P. putida was achieved under fed-batch conditions. According to the researchers the combination of computational modeling and strain engineering on P. putida for an advanced biofuels production has vital significance in enabling a bioproduction process that can use renewable carbon streams… read more. TECHNICAL ARTICLE
Genome-scale metabolic and pathway engineering… Credit: Metabolic Engineering, Volume 82, March 2024, Pages 157-170