Phys.org  May 9, 2024 Isoprenol is a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane) and is produced in several engineered microorganisms. Recently, Pseudomonas putida has gained interest as a future host for isoprenol bioproduction as it can utilize carbon sources from inexpensive plant biomass. A team of researchers in the US (industry, PNNL, Lawrence Berkeley National Laboratory) engineered metabolically versatile host P. putida for isoprenol production. They employed two computational modeling approaches to predict gene knockout targets and optimize the “IPP-bypass” pathway in P. putida to maximize isoprenol production. Altogether, the highest isoprenol production titer from P. putida was […]